Quantum description and properties of electrons emitted from pulsed nanotip electron sources

We present a quantum calculation of the electron degeneracy for electron sources. We explore quantum interference of electrons in the temporal and spatial domain and demonstrate how it can be utilized to characterize a pulsed electron source. We estimate effects of Coulomb repulsion on two-electron interference and show that currently available nano tip pulsed electron sources operate in the regime where the quantum nature of electrons can be made dominant

Regulation of Synaptic Pumilio Function by an Aggregation-Prone Domain

We identified Pumilio (Pum), a Drosophila translational repressor, in a computational search for metazoan proteins whose activities might be regulated by assembly into ordered aggregates. The search algorithm was based on evolutionary sequence conservation patterns observed for yeast prion proteins, which contain aggregation-prone glutamine/asparagine (Q/N)-rich domains attached to functional domains of normal amino acid composition. We examined aggregation of Pum and its nematode ortholog PUF-9 by expression in yeast. A domain of Pum containing the Q/N-rich sequence, denoted as NQ1, the entire Pum N terminus, and the complete PUF-9 protein localize to macroscopic aggregates (foci) in yeast. NQ1 and PUF-9 can generate the yeast Pin+ trait, which is transmitted by a heritable aggregate. NQ1 also assembles into amyloid fibrils in vitro. In Drosophila, Pum regulates postsynaptic translation at neuromuscular junctions (NMJs). To assess whether NQ1 affects synaptic Pum activity in vivo, we expressed it in muscles. We found that it negatively regulates endogenous Pum, producing gene dosage-dependent pum loss-of-function NMJ phenotypes. NQ1 coexpression also suppresses lethality and NMJ phenotypes caused by overexpression of Pum in muscles. The Q/N block of NQ1 is required for these phenotypic effects. Negative regulation of Pum by NQ1 might be explained by formation of inactive aggregates, but we have been unable to demonstrate that NQ1 aggregates in Drosophila. NQ1 could also regulate Pum by a "dominant-negative" effect, in which it would block Q/N-mediated interactions of Pum with itself or with cofactors required for translational repression

Programmable telemetry system Patent

Time division multiplexed telemetry transmitting system controlled by programmed memor

Chiral molecules split light: Reflection and refraction in a chiral liquid

A light beam changes direction as it enters a liquid at an angle from another medium, such as air. Should the liquid contain molecules that lack mirror symmetry, then it has been predicted by Fresnel that the light beam will not only change direction, but will actually split into two separate beams with a small difference in the respective angles of refraction. Here we report the observation of this phenomenon. We also demonstrate that the angle of reflection does not equal the angle of incidence in a chiral medium. Unlike conventional optical rotation, which depends on the path-length through the sample, the reported reflection and refraction phenomena arise within a few wavelengths at the interface and thereby suggest a new approach to polarimetry that can be used in microfluidic volumes

The Effects of Negative Legacies on the Adjustment of Parentally Bereaved Children and Adolescents

This is a report of a qualitative analysis of a sample of bereaved families in which one parent died and in which children scored in the clinical range on the Child Behavior Check List. The purpose of this analysis was to learn more about the lives of these children. They were considered to be at risk of developing emotional and behavioral problems associated with the death. We discovered that many of these “high risk” children had a continuing bond with the deceased that was primarily negative and troubling for them in contrast to a comparison group of children not at risk from the same study. Five types of legacies, not mutually exclusive, were identified: health related, role related, personal qualities, legacy of blame, and an emotional legacy. Coping behavior on the part of the surviving parent seemed to make a difference in whether or not a legacy was experienced as negative

Ionization Mechanisms in Jet-Dominated Seyferts: A Detailed Case Study

For the past 10 years there has been an active debate over whether fast shocks play an important role in ionizing emission line regions in Seyfert galaxies. To investigate this claim, we have studied the Seyfert 2 galaxy Mkn 78, using HST UV/optical images and spectroscopy. Since Mkn 78 provides the archetypal jet-driven bipolar velocity field, if shocks are important anywhere they should be important in this object. Having mapped the emission line fluxes and velocity field, we first compare the ionization conditions to standard photoionization and shock models. We find coherent variations of ionization consistent with photoionization model sequences which combine optically thick and thin gas, but are inconsistent with either autoionizing shock models or photoionization models of just optically thick gas. Furthermore, we find absolutely no link between the ionization of the gas and its kinematic state, while we do find a simple decline of ionization degree with radius. We feel this object provides the strongest case to date against the importance of shock related ionization in Seyferts.Comment: 4 pages, 1 figure, to appear in the proceedings of IAU Symposium 222 "The Interplay among Black Holes, Stars and ISM in Galactic Nuclei", T. Storchi Bergmann, L.C. Ho & H.R. Schmitt, ed

Distinct metabolic programs induced by TGF-β1 and BMP2 in human articular chondrocytes with osteoarthritis

Objectives: Cellular energy metabolism is important for the function of all tissues, including cartilage. Recent studies indicate that superficial and deep subpopulations of articular chondrocytes (ACs) have distinct metabolic profiles. At the cellular and molecular level, osteoarthritis (OA) is characterised by alteration from a healthy homoeostatic state towards a catabolic state. Several molecular pathways, including transforming growth factor beta (TGF-β) and bone morphogenetic protein (BMP) signalling, have been identified as critical players in the pathogenesis and progression of OA. However, the manner in which these factors influence cellular energy metabolism in ACs is not well understood. This study investigates the effect of TGF-β or BMP signalling on energy metabolism in human articular chondrocytes (hACs). Methods: ACs were isolated from residual macroscopically full thickness and intact cartilage from the femoral condyle of human samples obtained from patients with OA. ACs were treated with Vehicle (control), TGF-β1 or BMP2 for 48–72 hours. Metabolic assays were performed to determine glucose consumption, lactate production and adenosine triphosphate (ATP) production, whereas the mitochondrial stress test was performed to determine oxygen consumption rate. Protein was isolated to assess translational activity and was evaluated using Western blot. Results: We showed that TGF-β1, known to maintain chondrocyte homoeostasis, stimulated glycolysis by upregulating key glycolytic factors, such as glucose transporter 1 (Glut1) and hexokinase II, while reducing oxidative phosphorylation in hACs. In contrast, BMP2 enhanced mitochondrial metabolism and oxidative phosphorylation and had a minimal effect on key glycolytic regulators. Conclusions: Our data revealed distinct metabolic programs induced by TGF-β1 and BMP2 in hACs, suggesting that the regulation of cellular metabolism may represent a new mechanism underlying the pathogenesis of OA. The translational potential of this article: The findings define the regulation of energy metabolism as a potential novel therapeutic approach for the treatment of OA

Possible detection of singly-ionized oxygen in the Type Ia SN 2010kg

We present direct spectroscopic modeling of 11 high-S/N observed spectra of the Type Ia SN 2010kg, taken between -10 and +5 days with respect to B-maximum. The synthetic spectra, calculated with the SYN++ code, span the range between 4100 and 8500 \r{A}. Our results are in good agreement with previous findings for other Type Ia SNe. Most of the spectral features are formed at or close to the photosphere, but some ions, like Fe II and Mg II, also form features at ~2000 - 5000 km s$^{-1}$ above the photosphere. The well-known high-velocity features of the Ca II IR-triplet as well as Si II $\lambda$6355 are also detected. The single absorption feature at ~4400 \r{A}, which usually has been identified as due to Si III, is poorly fit with Si III in SN 2010kg. We find that the fit can be improved by assuming that this feature is due to either C III or O II, located in the outermost part of the ejecta, ~4000 - 5000 km s$^{-1}$ above the photosphere. Since the presence of C III is unlikely, because of the lack of the necessary excitation/ionization conditions in the outer ejecta, we identify this feature as due to O II. The simultaneous presence of O I and O II is in good agreement with the optical depth calculations and the temperature distribution in the ejecta of SN 2010kg. This could be the first identification of singly ionized oxygen in a Type Ia SN atmosphere.Comment: Submitted to MNRA

Field Work Reflections: Journeys in Knowing and Not-Knowing

In this paper, I retrace my interest in narrative forms of inquiry. I begin by revisiting a series of research projects that I conducted early in my career, describing some of my own dissatisfactions with the methods I used at the time. I move on to a detailed reexamination of my first piece of narrative research, completed during my PhD. In that project I used a narrative pointed psychosocial method in an attempt to develop new knowledge in the field of drugs, ‘race’ and ethnicity. In the final section, I consider what I have learned from this approach in terms of knowing and not-knowing and how I have used this experience to explore different approaches to narrative inquiry. I finish by drawing out some lessons I have learned from these different studies, which I hope might be of relevance to other social work researchers

X-ray Emission from the Radio Jet in 3C 120

We report the discovery of X-ray emission from a radio knot at a projected distance of 25" from the nucleus of the Seyfert galaxy, 3C 120. The data were obtained with the ROSAT High Resolution Imager (HRI). Optical upper limits for the knot preclude a simple power law extension of the radio spectrum and we calculate some of the physical parameters for thermal bremsstrahlung and synchrotron self-Compton models. We conclude that no simple model is consistent with the data but if the knot contains small regions with flat spectra, these could produce the observed X-rays (via synchrotron emission) without being detected at other wavebands.Comment: 6 pages latex plus 3 ps/eps figures. Uses 10pt.sty and emulateapj.sty. Accepted for publication in the ApJ (6 Jan 99